{"id":2255,"date":"2025-09-26T11:04:44","date_gmt":"2025-09-26T04:04:44","guid":{"rendered":"https:\/\/www.its.ac.id\/publikasi\/?p=2255"},"modified":"2025-09-26T11:04:44","modified_gmt":"2025-09-26T04:04:44","slug":"optimization-of-authentication-methods-for-processed-chicken-meat-products-based-on-nd5-gene-qpcr-2","status":"publish","type":"post","link":"https:\/\/www.its.ac.id\/publikasi\/en\/2025\/09\/26\/optimization-of-authentication-methods-for-processed-chicken-meat-products-based-on-nd5-gene-qpcr-2\/","title":{"rendered":"Optimization of Authentication Methods for Processed Chicken Meat Products Based on ND5 Gene qPCR"},"content":{"rendered":"<section class=\"item authors\">\n<ul class=\"authors\">\n<li><span class=\"name\">Afifatul Achyar <\/span><span class=\"affiliation\">Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang<\/span><\/li>\n<li><span class=\"name\">Fira Safitri <\/span><span class=\"affiliation\">UPT Laboratorium Terpadu UNP, Universitas Negeri Padang, Padang<\/span><\/li>\n<li><span class=\"name\">Yusni Atifah <\/span><span class=\"affiliation\">Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang<\/span><\/li>\n<li><span class=\"name\">Siska Alicia Farma <\/span><span class=\"affiliation\">UPT Laboratorium Terpadu UNP, Universitas Negeri Padang, Padang<\/span><\/li>\n<li><span class=\"name\">Dwi Hilda Putri <\/span><span class=\"affiliation\">Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang<\/span><\/li>\n<li><span class=\"name\">Yuni Ahda <\/span><span class=\"affiliation\">Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang<\/span><\/li>\n<\/ul>\n<\/section>\n<section class=\"item doi\">\n<h2 class=\"label\">DOI:<\/h2>\n<p><span class=\"value\"><a href=\"https:\/\/doi.org\/10.12962\/j22759970.v5i2.1823\">https:\/\/doi.org\/10.12962\/j22759970.v5i2.1823<\/a><\/span><\/p>\n<\/section>\n<section class=\"item keywords\">\n<h2 class=\"label\">Keywords:<\/h2>\n<p><span class=\"value\">qPCR, Authentication, Gallus, ND5 Gene, Chicken Nuggets<\/span><\/p>\n<\/section>\n<section class=\"item abstract\">\n<h2 class=\"label\">Abstract<\/h2>\n<p>Consumers have the right to correct information about the products they consume. One example of a food product that is vulnerable to counterfeiting is chicken nuggets. To overcome this problem, qPCR can be an effective solution. Previous research has designed primers targeting the chicken\u00a0<em>ND5\u00a0<\/em>gene. However, the PCR conditions for this primer pair have never been optimized. This research aims to optimize qPCR conditions and carry out chicken nugget authentication trials. Genomic DNA was isolated from five weight variations of chicken meat samples as a qPCR standard curve and three chicken nugget samples. Optimizing the annealing temperature with gradient PCR gave the best results at a temperature of 57.6<sup>O<\/sup>C. The results of the authentication test showed that the chicken meat content in TD nuggets was 54.7%, C nuggets 43.8%, and D nuggets 37.3%. This value is still in accordance with the Indonesian National Standard (SNI).<br \/>\n<a href=\"https:\/\/journal.its.ac.id\/index.php\/hr\/article\/view\/1823\">PDF<\/a><\/p>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>Afifatul Achyar Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang Fira Safitri UPT Laboratorium Terpadu UNP, Universitas Negeri Padang, Padang Yusni Atifah Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang Siska Alicia Farma UPT Laboratorium Terpadu UNP, Universitas Negeri Padang, Padang Dwi Hilda Putri Department of Biology, [&hellip;]<\/p>\n","protected":false},"author":250,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_joinchat":[],"footnotes":""},"categories":[212],"tags":[],"class_list":["post-2255","post","type-post","status-publish","format-standard","hentry","category-halal-research-journal"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - 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