Comparative Study of DNA Extraction Methods for Halal Food Testing: Advantages, Disadvantages, and Recommended Methods
DOI:
Keywords:
Food, Consumption, DNA Extraction, Halal Food, Comparative Study
Abstract
Identification of the halal status of food is a crucial aspect of the food industry, particularly in countries with a majority Muslim population. DNA extraction methods play a critical role in ensuring the halal status of food products by accurately detecting nonhalal substances. This study conducted a comparative evaluation of various DNA extraction methods used in halal food testing. We reviewed several DNA extraction methods listed in RSNI3 ISO 21571:2005 titled “Analytical Methods for the Detection of Genetically Engineered Products and Their Derivatives—Nucleic Acid Extraction”. In RSNI3 ISO 21571:2005, five DNA extraction methods are discussed: the phenol-chloroform method, the silica method, the CTAB method, the polyvinylpyrrolidone (PVP) method, and the guanidine chloroform method. This study analyzes the advantages and disadvantages of the literature review. Additionally, we conducted an economic and process effectiveness review to identify the best method. Based on this analysis, we recommend the most suitable method for testing the halal status of various types of food sample. The results of this study are expected to serve as a guideline for testing laboratories and the food industry in selecting the optimal DNA extraction method for ensuring efficient and accurate halal status of products.
PDF
Related News
-
A Halal-Haram Studies: Food-Grade Lubricants in the Food Industry
Nurul Huda Fauzan Naufal Dian Institut Teknologi Sepuluh Nopember Syah Sultan Ali Muzakhar Institut Teknologi Sepuluh Nopember Fahmi Fahmi
September 26, 2025 11:09 -
Optimization of Authentication Methods for Processed Chicken Meat Products Based on ND5 Gene qPCR
Afifatul Achyar Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang Fira Safitri UPT Laboratorium Terpadu
September 26, 2025 11:09 -
Comparative Study of DNA Extraction Methods for Halal Food Testing: Advantages, Disadvantages, and Recommended Methods
Citra Yulia SariInstitut Teknologi Sepuluh Nopember Raditya Yudhi PamungkasaProcess Technology Study Program, Department of Chemical Engineering, Faculty of Industrial
September 26, 2025 11:09 -
Policy Evaluation Of The Halal Product Guarantee Agency (BPJPH): Impact On Producer Compliance
Anisa Tul Fitri Universitas Islam Negeri Imam Bonjol Padang Ahmad Wira Universitas Islam Negeri Imam Bonjol Padang Aidil Novia
September 26, 2025 11:09
